Aktuális szakdolgozati témák
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Téma címe |
Téma rövid leírása |
Szakirodalomazás (SZ)/Kísérletes (K) |
Kontaktszemély, elérhetőség |
| Fluoreszcensen jelölt plazmid alkalmazása spórafelszíni fehérjék lokalizációs mintázatának vizsgálatára/Application of fluorescently tagged plasmids for investigating the localization patterns of spore surface proteins (magyar/english) | he CotH family comprises a group of cell surface-associated proteins originally identified in bacterial spore coats, but later found to be expanded in several fungal lineages, particularly in Mucorales and are predicted to play a role in spore wall integrity, host-pathogen interactions, and virulence. In this study, plasmid constructs encoding fusion proteins of spore surface-associated candidates (e.g., CotH family proteins) with fluorescent reporters (e.g., mCherry) are generated and introduced to the fungus via transformation methods. | SZ/K |
Prof. Dr. Tamás Papp Dr. Szebenyi Csilla |
| Targeting the cotH gene family as a novel immunotherapeutic strategy for the treatment of mucormycosis (english). | Given their surface localization and essential role in host cell invasion, CotH proteins represent attractive targets for immunotherapeutic intervention in mucormycosis. Monoclonal antibodies or peptide inhibitors targeting CotH-integrin or CotH-GRP78 interactions have demonstrated protective effects in preclinical models. CotH proteins are immunogenic and could serve as components of fungal vaccines or diagnostic markers. Targeting the CotH gene family offers a promising, fungus-specific strategy that could enhance host immunity and improve outcomes in immunocompromised patients at high risk of mucormycosis. | SZ |
Prof. Dr. Tamás Papp Dr. Szebenyi Csilla |
| A cotH géndeléció hatása a Mucor lusitanicus spórafalának összetételére analitikai módszerekkel. Polysaccharide composition of the spore wall in filamentous fungi and its role in immune evasion (magyar/english). | The spore wall of filamentous fungi consists of a dynamic network of polysaccharides. These structural components not only confer mechanical resilience to spores but also play a crucial role in modulating host immune responses. The CotH proteins that play key roles in host interaction and fungal virulence in Mucorales. In Mucor lusitanicus, disruption of cotH genes may have profound effects on spore wall architecture and biochemical composition, which in turn influence immune recognition and pathogenicity. This study aims to investigate how cotH deletions alter the polysaccharide and protein constituents of the spore wall using advanced analytical methods. | K |
Prof. Dr. Tamás Papp Dr. Szebenyi Csilla |
| Diagnostic potential of cotH and hsbA genes as biomarkers for mucormycosis (english). | Due to their high conservation within Mucorales and absence in other fungal groups, cotH genes have emerged as highly specific molecular biomarkers for mucormycosis. Quantitative PCR assays targeting cotH sequences allow for rapid, non-culture-based detection of Mucorales DNA in clinical samples such as serum or bronchoalveolar lavage fluid, often enabling earlier diagnosis than traditional histopathology or culture. The functional role and diagnostic or therapeutic potential of hsbA genes remain unclear and require further investigation. | SZ |
Prof. Dr. Tamás Papp Dr. Szebenyi Csilla |
| A Mucorales spórák immunelkerülő mechanizmusai. Immune evasion mechanisms of Mucorales spores (magyar/english). | Mucorales spores responsible for mucormycosis have evolved sophisticated immune evasion strategies that facilitate host invasion, particularly in immunocompromised individuals. These immune evasion mechanisms allow Mucorales spores to establish infection in vulnerable hosts, highlighting the need for early detection and novel immunotherapeutic strategies. | SZ/K |
Prof. Dr. Tamás Papp Dr. Szebenyi Csilla |
| A Ricin-szerű toxint (RLT) kódoló gének molekuláris karakterizálása Mucor lusitanicus-ban. Molecular characterization of ricin-like toxin (RLT)-encoding genes in Mucor lusitanicus (magyar/english) | RLTs are known for their potent cytotoxic effects. Functional studies using CRISPR-Cas9 generated knockout or overexpression strains aim to elucidate the potential role of RLTs in host cell damage, immune evasion, and fungal virulence, offering insights into novel pathogenicity mechanisms in mucormycosis. | K |
Prof. Dr. Tamás Papp Dr. Szebenyi Csilla |
| A CotH és HsbA gének immunmodulációs szerepének vizsgálata Mucor lusitanicus mutánsok esetében HaCaT keratinocita modellen, ELISA alapú cytokinvizsgálattal. Investigating the immunomodulatory role of CotH and HsbA proteins in Mucor lusitanicus mutants using a HaCaT keratinocyte model and ELISA-based cytokine asssays (magyar/english) | CotH and HsbA proteins are implicated in host-pathogen interactions and stress response. This study utilizes targeted Mucor lusitanicus mutants deficient in specific cotH and hsbA genes to assess their role in modulating host immune responses. HaCaT human keratinocyte cells serve as an in vitro model of epithelial interaction. Following fungal exposure, ELISA-based cytokine profiling is performed to quantify inflammatory responses. Comparative analyses between wild-type and mutant strains can identify fungal factors that suppress or enhance cytokine production. | K |
Prof. Dr. Tamás Papp Dr. Szebenyi Csilla |
| Deléciós mutánsok létrehozása és azok karakterizálása Mucor lusitanicus-ban CRISPR-Cas9 rendszer segítségével/Generation and characterization of deletion mutants in Mucor lusitanicus using the CRISPR-Cas9 system (magyar) | A kutatás célja a Mucor lusitanicus deléciós mutánsainak létrehozása a CRISPR-Cas9 rendszer alkalmazásával, valamint ezek fenotípusos és molekuláris jellemzése. A vizsgálat hozzájárul a gomba genetikai hátterének és patogenitásban betöltött szerepének jobb megértéséhez. The aim of this study is the generation of Mucor lusitanicus deletion mutants using the CRISPR-Cas9 system, followed by their phenotypic and molecular characterization. This approach provides valuable insights into the genetic background of the fungus and its potential role in pathogenicity. | K |
Prof. Dr. Tamás Papp Dr. Szebenyi Csilla |
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